Apoptosis upon targeted ablations.
Whole-mount TUNEL staining of 3 to 4ss (A–D) and 8 to 9ss (I–L) control (A, I), Nkx2.1:Cre;Dbx1DTA (B, J), Foxg1Cre;Dbx1DTA (C, K) and Wnt1:Cre;Dbx1DTA (D, L) embryos. E–H and M–P are cryosections of the embryos shown in A–D and I–L collected at the level indicated by a dashed line. Neural tissues are surrounded by a dashed line for better visualisation. (Q–T) Immunostaining for activated Caspase-3 (red), YFP (green) and DAPI (blue) on coronal cryosections of Nkx2.1:Cre;R26YFP;Dbx1DTA (Q), Foxg1Cre;R26YFP;Dbx1DTA (R), Wnt1:Cre;R26YFP;Dbx1DTA (S) and control (T) embryos. Images in Q–T correspond to a single confocal plane. Arrows point to Caspase-3+/YFP+ cells, arrowheads indicate Caspase-3+/YFP− cells. Scale bars: A, I: 200 µm; E, M, Q, R: 100 µm; S, T: 50 µm.