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Antiproliferative and apoptotic effects of sorafenib.

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posted on 19.04.2013 by Carmelo Carlo-Stella, Silvia L. Locatelli, Arianna Giacomini, Loredana Cleris, Elena Saba, Marco Righi, Anna Guidetti, Alessandro M. Gianni

Following incubation with increasing doses of sorafenib, viable cell counts and apoptotic cell death were assessed by annexin-V/PI double staining and flow cytometry analysis. (A) Viable cell counts upon exposure to 0 (▪), 5 (□) and 10 (•) µM sorafenib. Viable cells are expressed as fold increase (FI) of annexin-V/PI cells after 24 and 48 hours of incubation with sorafenib as compared to day 0. (B) Cell death following 24 (□) or 48 (▪) hours sorafenib exposure. Percentages of cell death include annexin-V+/PI plus annexin-V+/PI+ plus annexin-V/PI+ cells. Values refer to four independent experiments. * p≤0.001, ° p≤0.01, and ≠ p≤0.05, compared to controls.