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Antibacterial activity and agglutination.

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posted on 10.04.2014, 04:07 authored by Miti Jearaphunt, Chadanat Noonin, Pikul Jiravanichpaisal, Seiko Nakamura, Anchalee Tassanakajon, Irene Söderhäll, Kenneth Söderhäll

The number of E. coli after in vitro incubation with recombinant proPO fragments or GFP as a control, compared with a buffer control (A), was determined. All experiments were repeated at least three times (N = 3). Each bar represents the mean ± SEM, and *** P<0.001 indicates significant differences between the treatment and Tris-HCl. The data were analyzed by one-way ANOVA. E. coli observed by light microscopy after treatment with the recombinant fragments. The scale bars represent 10 µm (B). Changes in bacterial morphology after treatment with the proPO-ppA fragment as observed by SEM (C–J). After 15 min of incubation with proPO-ppA (C) or GFP (D), cell wall disruption started to appear and is reflected by longitudinal lines (black arrows in C) in the proPO-ppA treatment. The scale bars represent 1 µm. After 40 min of incubation with Tris-HCl (E), proPO-ppA (F), and GFP (G), agglutination was clearly observed in the proPO-ppA samples. The scale bars represent 10 µm. The E. coli cells were clearly distorted after proPO-ppA treatment (H–I) in contrast to the GFP treatment (J). The pictures shown in (H) and (I) were taken from different areas. The scale bars represent 1 µm.