Anti-Leishmania antibodies in accordance to Leishmania load in the spleen.
A. Total anti-Leishmania IgG antibodies. B. Anti rK-39 antibodies. The cut-off values of 0.096 and 0.112 for SLA and rK-39, respectively, which corresponded to the mean of the results of five healthy dogs plus three standard deviations. The dashed line represents the cut off value for the test. C and D levels of IgG1 and IgG2 subclasses. Analysis was performed using One-way ANOVA or Kruskal-Wallis test. The Kolmogorov-Smirnov test was used to test the normality of the data.