Angiotensinogen protein expression in WKY and SHR myocytes.
Figures are generally photos, graphs and static images that would be represented in traditional pdf publications.
Western blotting of angiotensinogen (AOGEN) protein expression in neonatal WKY and SHR myocytes (maintained for 48 hours in serum-deprived medium) by using an antibody directed against an epitope on the NH2-terminus region of the angiotensinogen protein (residues 44–56). The myocytes cell lysate (50 µg protein) were separated by gel electrophoresis and transferred on PVDF. Equal protein loading was confirmed by EF-1α detection in cell lysates. Relative O.D. (AOGEN/EF-1 α) is represented as the mean ± SE of three samples from each group.