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Analysis of the protein expressed in E. coli BL21 cells following purification on a 12% SDS-PAGE.

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posted on 2015-03-06, 09:47 authored by Junmei Ding, Chaofan Wang, Zhenrong Xie, Junjun Li, Yunjuan Yang, Yuelin Mu, Xianghua Tang, Bo Xu, Junpei Zhou, Zunxi Huang

Lane M, protein molecular marker; Lane 1, before induction with 0.7 mM IPTG; Lane 2, after induction with IPTG and grown at 20°C for 20 h; and Lane 3, purified recombinant CarEW (∼53.76 kDa).