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Analysis of the difference between T2A, E2A and F2A on EGFP expression by cells.

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posted on 19.02.2013, 20:53 by Steven Y. Gao, Michelle M. Jack, Christopher O’Neill

(A) Replacing F2A peptide with T2A peptide in a two-gene construct caused a significant increase in mean EGFP expression per positive cell. (B) Replacing E2A with T2A in a two-gene construct caused a significant increase in the mean EGFP expression per positive cell. (C) In a three-gene construct, the expression of EGFP was not altered after replacing either a single single F2A or E2A with T2A. A significant increase in EGFP expression was detected when both F2A and E2A were replaced with T2A. Data presented as mean ± SEM from 3 independent experiments. AU  =  arbitrary units.

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