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Analysis of the cooperation between β-catenin activation and Igf2 overexpression in 10 month-old mice. A- Igf2 overexpression in 10 month-old ΔCat mice does not significantly alter the adrenal phenotype.

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posted on 28.08.2012, 01:47 by Coralie Drelon, Annabel Berthon, Bruno Ragazzon, Frédérique Tissier, Roberto Bandiera, Isabelle Sahut-Barnola, Cyrille de Joussineau, Marie Batisse-Lignier, Anne-Marie Lefrançois-Martinez, Jérôme Bertherat, Antoine Martinez, Pierre Val

AdIgf2 mice were mated with ΔCat mice to generate ΔCat;AdIgf2 compound animals. The adrenal phenotype in 10 month-old mice was analysed by haematoxylin & eosin staining (a–c) and immunohistochemistry for Dab2. Black arrows show infiltrating subcapsular mesenchymal cells. B- Analysis of Ki67 expression in 10 month-old ΔCat;AdIgf2 adrenals. Ki67 expression was analysed by immunohistochemistry in wild-type (B, a), ΔCat (B, b) and ΔCat;Igf2 (B, c) adrenals. C- Igf2 overexpression in ΔCat mice does not significantly increase proliferation. a-Counting of Ki67-positive cells. Numbers of Ki67-positive cells in a whole adrenal section were counted separately in the cortex (Co) and medulla (M). For each zone, the number of cells was corrected for surface and is expressed as a percentage of Ki67-positive cells in one of the control individuals. Bars represent the mean of at least 7 individual counts in wild-type, ΔCat and ΔCat;AdIgf2 adrenals ± standard deviation. P-value was calculated using Student's t-test. b- Analysis of Cyclin D1 expression. Cyclin D1 expression was analysed by RTqPCR with cDNAs from wild-type, ΔCat and ΔCat;AdIgf2 adrenals. Bars represent the mean relative quantification (Rq Tg/WT) of gene expression in at least 7 adrenals per genotype ± standard deviation. P-value was calculated using Student's t-test. D- Analysis of malignancy markers expression. Expression of VegfA, Connexina43 (cnx43) and Nov/Ccn3 was analysed by RTqPCR with cDNAs from wild-type (WT), ΔCat and ΔCat;AdIgf2 adrenals. Bars represent the mean relative quantification (Rq Tg/WT) of gene expression for each marker in at least 7 adrenals per genotype ± standard deviation. P-value was calculated using Student's t-test. M, medulla; F, fasciculata; G, glomerulosa. Scale bar is 80 µm.

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