Analysis of the canonical Wnt signaling pathway in adipose tissue from wild-type mice and adiponectin-transgenic mice at the age of 20 weeks.
(A) Western blot analysis of total β-catenin in whole-cell extracts and non-phosphorylated (active form) β-catenin in nuclear extracts. (B) Relative protein levels of total β-catenin in whole-cell extracts and non-phospho-β-catenin in nuclear extracts of adipose tissue from transgenic mice shown as ratios to those of wild-type mice (n = 5, each). (C) mRNA expression of c-Myc (Myc) and cyclin D1 (Ccnd1) genes in transgenic mice shown as ratios to wild-type mice (n = 10, each). Means and SD.