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Analysis of anti-FaASR titer by ELISA.

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posted on 2011-09-06, 02:45 authored by Jian-ye Chen, Du-juan Liu, Yue-ming Jiang, Ming-lei Zhao, Wei Shan, Jian-fei Kuang, Wang-jin Lu

The antibody at different dilutions (400- to 819,800-fold) was reacted with the equal amount of the purified fusion protein (1 μg). Antibody titer is defined as the dilution of the antibody corresponding to an absorbance of 0.500 at 490 nm. The arrow indicated the 0.500 absorbance value that corresponds to antibody dilution times of about 25,000. Values in the figure were the means ± S.D. (n = 3).

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