Analysis of SV and active zone markers.

(A) XY plot comparing changes in punctal fluorescence for SV markers: RAB-3 vs. SNB-1 synaptobrevin across mutants tested. Solid orange circles indicate mutants that are apparent outliers described in the text. (B) Images of RAB-3 in axons of wild type and the indicated mutant animals. Quantification of RAB-3 punctal fluorescence is shown to the right. Scale bar, 5 µm. (C) Images of the SNB-1 synaptobrevin in axons of wild type and the indicated mutant animals. Quantification of SNB-1 synaptobrevin punctal fluorescence is shown to the right. (D) Images of SYD-2 α-Liprin in axons of wild type and the indicated mutant animals. Quantification of SYD-2 α-Liprin punctal fluorescence is shown to the right. In (B–D), ** indicates p<0.01, (Student's T-Test compared to wild type). All error bars are ±SEM. Separate charts indicate data from separate sets of experiments.