Analysis of PK-resistant PrPSc in Tg(M109) mice inoculated with diverse prion isolates.
Except for one undigested lane (−), brain samples were digested with PK (+ in panel A). All inoculation experiments resulted in the generation of PK-resistant PrPSc. (A) Detergent-extracted brain homogenates from Tg(M109) mice that were inoculated with the indicated prion isolates. Brain homogenate from an uninoculated, 97-d-old mouse (None) is shown as a control. (B–K) PK-resistant PrPSc in the inocula (Inoc.), and after 1st and 2nd passages in Tg(M109) mice. Inocula were sCJD(MM1) (B); sCJD(MM2) (C); sCJD(VV2) (D); vCJD (E); BSE (F); CWD (G); scrapie SSBP/1 (H); Sc237 (I); RML (J); 301V(A) or 301V(B) (K) prions. With the exception of 301V, the electrophoretic mobility of PK-resistant PrPSc for each strain following passage in Tg(M109) mice was similar to the original isolate. In all panels, loading quantities were adjusted prior to immunoblotting to give similar signal intensities across all samples. Molecular weight measurements are shown in kDa. PrP was detected using the antibody HuM-P.