Analysis of CPY* export signals.
(A) Schematic representation of CPY* and its deletion variants (D1–D6). Carbohydrate chains are shown by branched symbols, asterisks indicate the position of the G255R mutation, dark shaded boxes indicate signal sequences, and the HA epitope tag is shaded light gray. (B) Intracellular localization of highly expressed CPY* deletion variants in wild type and Δpep4 strains. CPY* variants was detected using anti-HA antibody and visualized in the green channel. ER and nuclear envelope was visualized in the red channel using anti-Kar2p antiserum. (C) Intracellular localization of D1 and D2 variants in Δpep4 cells. Substrates and ER/nuclei are visualized as in panel B. Localization of all CPY* deletion variants in both wild type and Δpep4 cells are shown in Figure S1. Arrowhead indicates the accumulated CPY* or its variant in vacuole in a representative cell. Scale bars, 5 µm.