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Analysis in strains that over-express Puf3p.

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posted on 2013-02-20, 17:57 authored by Marc Chatenay-Lapointe, Gerald S. Shadel

Shown is a wild-type strain with a plasmid that over-expresses Puf3p (PUF3) or the corresponding empty vector (vector). (A) Mitochondrial oxygen consumption during early and late log phase. (B) Western blot analysis of protein extracts derived from late log cultures (representative of three biological replicates). (C) Steady-state levels of COX17, COX12, and RIP1 transcripts in late log phase. The values indicate the mean +/− SD with p-values indicated as described in the legend of Figure 1.

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