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An active paxe promoter is required for axe-txe mediated stable plasmid maintenance.

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posted on 2013-09-03, 02:16 authored by Lidia Boss, Łukasz Labudda, Grzegorz Węgrzyn, Finbarr Hayes, Barbara Kędzierska

Stability assays were conducted with derivatives of the stability probe vector, pFH450: pREGΔaxe-txe does not contain any accessory stability determinants (circles), pREG531 contains the axe-txe cassette (squares), and pREGpaxemut contains the axe-txe cassette with a mutated paxe promoter (triangles). Assays were performed as outlined in Materials and Methods. Results are averages of at least five experiments for which the standard deviation did not exceed 15%.


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