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Allopurinol and uricase pre-treatment attenuate alveolar barrier dysfunction.

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posted on 30.11.2012, 00:55 by Maria T. Kuipers, Hamid Aslami, Alexander P. J. Vlaar, Nicole P. Juffermans, Anita M. Tuip-de Boer, Maria A. Hegeman, Geartsje Jongsma, Joris J. T. H. Roelofs, Tom van der Poll, Marcus J. Schultz, Catharina W. Wieland

Mice were pre-treated with vehicle (10% dimethylsulfoxide, dark grey bars), uricase (0.2 mg/kg, light grey, striped bars), or allopurinol (25 mg/kg (white bars) 1 hour before start of mechanical ventilation (VILI). Spontaneously breathing, vehicle pre-treated mice served as controls (C). After 5 hours mice were killed. Lung wet-to-dry ratio (A), total protein levels in lung lavage fluid (B) and immunoglobulin M (IgM) concentrations in (C) were analyzed. Data represent mean ± SEM of 4 control mice and n = 9 for the VILI groups (for IgM n = 5−6). ##P<0.01, ###P<0.001 vs. vehicle ventilated. ***p<0.001 vs. vehicle control.