All-point current histogram for KKRR-ShPrP(120–232) and KKRR-ShPrP(120–232)-D178N.

Ionic current histogram of all PrP^C capture events from all voltages, with ionic current normalized by the open-pore current (I0) at a given voltage, for KKRR-ShPrP(120–232) (blue) and KKRR-ShPrP(120–232)-D178N (red). Ionic current is median filtered to 2.99 ms per data-point. The histograms exhibit multiple peaks with varying degrees of overlap indicative of complex PrP^C kinetics in the pore. Moreover, the histograms exhibit clear distinguishable features (e.g. the near absence of the peak at I/I0 ∼0 pA with respect to KKRR-ShPrP(120–232)-D178N).