Figure 5.tif (1.2 MB)
Aligned amino acid sequence of 21 anti-Cap specific VHH antibody fragments.
figure
posted on 2013-03-02, 05:16 authored by Xiangjing Fu, Xiaolong Gao, Shengfang He, Di Huang, Peng Zhang, Xinglong Wang, Shuxia Zhang, Ruyi Dang, Shuanghui Yin, Enqi Du, Zengqi YangAmino acid sequences were aligned according to the Kabat numbering [16]. The dots denote the same sequences compared with VHH1. Differences in the sequence are shadowed, and the dash shows the missing sequences. The hallmark Cys residues are denoted by the thick-line boxes. The four conservative hallmark residues of VHH in FR2 (Val37Phe, Gly44Glu/Lys, Arg45Leu, and Trp47Gly) are denoted by the dotted line boxes.
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vivo VHH throughput screeningh eavy-chain antibodieCap-specific VHH sequencesCamelus Bactrianus VHH yeast two-hybridY 2H strategyporcine circovirus type 2enzyme-linked immunosorbent assayPCV 2VHH Y 2H libraryPorcine Circovirus Type 2Y 2H library screeningCamelid Single-Chain Antibody Yeast Two-Hybrid Library Produced De Novoh eavy chainPlate library construction systemY 2H libraryv ariable domain
