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Aldolase C and VEGF mRNA Expression at Different Oxygen Tensions

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posted on 22.02.2013, 08:41 authored by Thomas G Smith, Jerome T Brooks, George M Balanos, Terence R Lappin, D. Mark Layton, Dawn L Leedham, Chun Liu, Patrick H Maxwell, Mary F McMullin, Christopher J McNamara, Melanie J Percy, Christopher W Pugh, Peter J Ratcliffe, Nick P Talbot, Marilyn Treacy, Peter A Robbins

Lymphocytes were isolated from venous blood taken from CP patients and normal control participants, and incubated at eight different levels of oxygen tension prior to RNA isolation. Gene expression is shown relative to a standard calibrator sample. Basal gene expression at 20% oxygen was significantly higher in CP patients for both Aldolase C (A) and VEGF (B) ( p < 0.05). Both genes were induced by hypoxia, and at the lowest oxygen tension (0.1%) expression was no longer significantly different for either gene. Values are mean ± standard error of the mean. Asterisks indicate p < 0.05 (unpaired t-test).