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Affinity chromatographic purification of Rv1430 full-length protein, PE-PPE and Ser199Ala PE-PPE domains.

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posted on 2013-02-01, 01:41 authored by Rafiya Sultana, Mani Harika Vemula, Sharmishta Banerjee, Lalitha Guruprasad

Eluted fractions of the proteins recovered during the various purification steps were separated by SDS–12% polyacrylamide gel electrophoresis and stained with Coomassie brilliant blue R250. (A) Purified protein full-length Rv1430 (59.3 kDa); (B) Purified PE-PPE domain of Rv1430 (25.8 kDa); (C) Purified mutated PE-PPE domain of Rv1430 (25.8 kDa).