Affinity chromatographic purification of Rv1430 full-length protein, PE-PPE and Ser199Ala PE-PPE domains.

Eluted fractions of the proteins recovered during the various purification steps were separated by SDS–12% polyacrylamide gel electrophoresis and stained with Coomassie brilliant blue R250. (A) Purified protein full-length Rv1430 (59.3 kDa); (B) Purified PE-PPE domain of Rv1430 (25.8 kDa); (C) Purified mutated PE-PPE domain of Rv1430 (25.8 kDa).