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Activity of rationally constructed Sav-hybrid variants.

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posted on 07.09.2011, 02:23 by D. Dafydd Jones

The donor fragments at each of the 6 regions derives from one of the subtilisins, which is indicated in superscript in the figure. An active variant is indicated by the production of a clearing zone or halo around the B. subtilis colony due to digestion of casein embedded in the agar growth medium.