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Activation of transcription factor FKHRL1 by resveratrol.

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posted on 20.02.2013, 21:36 by Suthakar Ganapathy, Qinghe Chen, Karan P. Singh, Sharmila Shankar, Rakesh K. Srivastava

(A), Inhibition of FKHRL1 phosphorylation by resveratrol. Western blot analysis was performed to measure the expression of phospho-FKHRL1 in tumor tissues derived from control and/or drug treated mice on week 6. (B), Immunohistochemical examination of phospho-FKHRL1. Immunohistochemistry was performed to measure the expression of phospho-FKHRL1 in tumor tissues derived from control, resveratrol and/or TRAIL treated mice on week 6. (C), Quantification of FKHRL-positive cells in tumor tissues. (D), FKHRL1-DNA binding activity. Nuclear extracts were prepared from tumor tissues derived from control and drug-treated mice. Gelshift assay was performed to measure the FKHRL1-DNA binding activity as described in Materials and Methods.

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