Activation of ACS gene expression in Arabidopsis after B. cinerea infection.
(A) Twelve-day-old Arabidopsis seedlings grown in GC vials were inoculated with B. cinerea spores. Samples were collected at indicated times for total RNAs isolation. Expressions of all nine ACS genes were quantified by real-time PCR. Induction of ACS gene expression (fold of induction relative to the level before inoculation) was calculated by the double ΔCt method. (B) ACS transcript levels are expressed as percentage of EF1α transcript, which allows comparison of expression levels between different ACS genes. In both calculations, the expression of EF1α was used as a reference. Error bars indicate standard deviations (n = 3). ND, not detectable.