Actin depolymerization inhibits WASH recovery on endosomes after photobleaching.
(A) Stable 3T3 cells expressing GFP-WASH were treated as in Fig. 2A. WASH domains were photobleached and signal recovery was monitored for 3 min. A representative sequence of recovery is displayed (8 sec between frames). Scale bar: 1 µm. (B) Recovery of 72 WASH domains for 16 control cells and 98 WASH domains for 13 LatA-treated cells were pooled from 2 independent experiments to calculate the average (± s.e.m.) of WASH intensity at each time point.