Acetone extract of P. coriacea and physciosporin reduced RhoGTPase activity.
(A-B) The levels of GTP-bound Cdc42 (A) and Rac1 (B) were measured in A549 cells treated with 5 μg/mL of acetone extract of P. coriacea or physciosporin. GTP-Cdc42 and -Rac1 were measured using GST-PBD. The total amounts of Cdc42 and Rac1 were shown to measure relative activity. Quantitative data were obtained from three independent experiments, n = 3. Data represent the mean ± S.E.M. (standard error of the mean). ***p<0.001 compared to DMSO-treated A549 cells.