Absorption spectra of ThT incorporated into the insulin amyloid fibrils.
Panel a. Curve 1 – Superposition of the absorption spectra of free ThT (at concentration Cf), ThT bound to fibrils (at dye concentration Cb) and the apparent absorption caused by fibrils light scattering; Curve 2 – Apparent absorption caused by fibrils light scattering. Insert: Determination of the apparent absorption caused by fibrils light scattering: Dscat = aλ−m. Coefficients a and m were determined from the linear part of the curve 1 plotted in logarithmic coordinates lg(Dscat) = f(lg(λ)). Panel b. Curve 1 is the total absorption of free and bound ThT after light scattering subtraction. Curve 2 is the absorption spectra of free ThT. Curve 3 is the absorption spectra of the ThT incorporated into the amyloid fibrils – the difference between 1 and 2 spectra. Insulin concentration was 6.9·10−5 M.