Aberrant differentiation in the skin of RASSF9−/− mice—filaggrin (green).

(A) Frozen sections of dorsal skins of RASSF9−/− and WT control mice were immunostained with green fluorescence for filaggrin and red fluorescence for K14 in two-week-old mice. Blue, DAPI staining of cell nuclei. Scale bar  = 100 µm. (B) Images of higher magnification for filaggrin staining (green fluorescence) in skin sections. The dashed white lines denote the epidermis-dermis border. Similar results were obtained from three independent pairs of mice. Scale bar  = 20 µm; GL, granular layer; KL, keratinocyte layer. Note the increased thickness and patchiness in layer of prominent filaggrin staining, as well as abnormal distribution of filaggrin staining in keratinocyte layer and hair follicle (*).