A typical 3-D microfluidic tri-culture model.
(A) The microfluidic platform consists of 4 microchannels, each 500 μm in width, 75 μm in height and 2 cm in length. The divergent angles are 30°. Near the inlets and outlet there are perfusion channels. The enlarged boxed area illustrates multiple cell types embedded in a 3-D matrix. (B) Optical image of a microfluidic platform. (C) SEM images of top view (upper panel) and side view (lower panel) of the perfusion channels.