A subset of TuJ1(+) cells are proliferating.
Sections of OE harvested from E16.5 wild type animals were stained with TuJ1 and co-stained with phospho-histone 3 (pH3, A–C) or proliferating cell nuclear antigen (PCNA, D–F) and Hoechst 33258 to label nuclei. (A, D) Red channel only. (B, E) Merged images including Hoechst nuclear stain (blue). (C, F) Green channel only. Arrows indicate double positive cells. Scale bar in F is 25 µm and applies to all panels.