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A role of Vps4 in rLCMV-LASVGP and LCMV entry.

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posted on 20.02.2013, 15:01 by Giulia Pasqual, Jillian M. Rojek, Mark Masin, Jean-Yves Chatton, Stefan Kunz

(A) HEK293 cells were transiently transfected with FLAG-tagged wild-type Vps4A and the DN mutant Vps4AQE and expression of the recombinant proteins detected in Western-blot. (B) Expression of DN Vps4A reduced viral infection. Cells were transiently transfected with FLAG-tagged wild-type and DN Vps4A. After 36 hours, cells were infected with rLCMV-LASVGP and LCMV (MOI = 1). After 16 hours, cells were fixed and stained for the Vps4A variants (anti-FLAG) and LCMV NP. Cells were analyzed by FACS separating transfected “expressing” from untransfected “non-expressing” cells with gating based on the intensity of the anti-FLAG signal. The percentage of cells infected within each population was quantified. (C) Quantitation of (B). All data presented are means (n = 3 ± SD).