A large part of T cell signaling associates with the lamellum.

(A) 5C.C7 T cells expressing the indicated sensors were activated on peptide loaded CH27 APCs (10μM MCC) and percentage occurrence of each pattern of interface enrichment (Fig 1A)[3] among all cell couples analysed across multiple experiments is given in shades of red from -40 to 420 s. In addition, to address the rate of pattern change, the percentage change per 20-s interval was tabulated (C-40 to L300 in the bottom part of the figure). Red indicates an increase and green a decrease in the percentage occurrence of a pattern relative to the previous time point. The cluster tree is given in pink. The source data for this figure and the sensors used are listed in S1 Table. (B-D) Pattern classification graphs, similar to Fig 1E, are given for (B) GFP-SKAP55 (number of cell couples analyzed across multiple independent experiments, n = 59), (C) Myosin 1C-GFP (n = 56), (D) NFκB p65-GFP (n = 44, % cell couples with nuclear accumulation of NFκB are given in addition in grey). Error bars are s.e.m.