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A: Relative fluorescence intensity at 515 nm of SITS – labeled αA-crystallins interacting with LYI – labeled βL- crystallin.

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posted on 21.02.2013 by Anbarasu Kumarasamy, Edathara C. Abraham

Time – dependent increase in acceptor fluorescence in emission intensity is due to subunit exchange. Increase in the relative fluorescence intensity at 515 nm is due to energy transfer from the SITS – labeled protein to the LYI – labeled protein. SITS – labeled αA- wt (♦), αA1–172(▪), αA1–168 (▴) and αA1–162 (•) were incubated with LYI – labeled βL- crystallin. Each curve was analyzed with the best curve fit of the data to the exponential function Ft/F0 = A1+A2 e−kt. B: Relative fluorescence intensity at 426 nm of SITS – labeled αA-crystallins interacting with LYI – labeled βL- crystallin. Time – dependent decrease in donor fluorescence in emission intensity is due to subunit exchange. Decrease in the relative fluorescence intensity at 426 nm is due to energy transfer from the SITS – labeled protein to the LYI – labeled protein. SITS – labeled αA- wt (♦), αA1–172(▪), αA1–168 (▴) and αA1–162 (•) were incubated with LYI – labeled βL- crystallin. Each curve was analyzed with the best curve fit of the data to the exponential function Ft/F0 = A1+A2 e−kt.

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