(A) Extracted BAEC models were stretched or unstretched for 10 min and double labeled with 4G10 and anti–pan-cadherin

Note that cells in the stretched monolayer are more clearly outlined by 4G10 staining than those in the unstretched sample. No significant changes were observed in anti-cadherin staining before and after stretching. Bipolar arrow indicates the direction of stretch. Bar, 20 μm. (B) BAECs cultured on an elastic substrate were treated with Lipofectamine only (LF control) or transiently transfected with PECAM-1 siRNA and then extracted. The extracted models were stretched or left unstretched for 5 min at 37°C and their tyrosine phosphorylated protein levels determined using 4G10 immunoblotting. Arrow indicates a 130-kD band whose phosphorylation level was diminished in PECAM-1 siRNA transfected cells.

Copyright information:

Taken from "Mechanotransduction in an extracted cell model: Fyn drives stretch- and flow-elicited PECAM-1 phosphorylation"

The Journal of Cell Biology 2008;182(4):753-763.

Published online 25 Aug 2008

PMCID:PMC2518713.