(A) Exponentially-growing cells were incubated with 1, 2, or 10 μg/ml of LVFX for 10 min and then labeled with S-methionine and cysteine for 1 min at 30°C
Taken from "Effects of disruption of heat shock genes on susceptibility of to fluoroquinolones"
BMC Microbiology 2003;3():16-16.
Published online 12 Aug 2003
Copyright © 2003 Yamaguchi et al; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.Heat shock culture was prepared as described in Methods. (B) After incubation with 10 μg/ml of LVFX, aliquots (100 μl) of the cultures were taken at indicated times and labeled for 1 min at 30°C. Equal amounts of acid-precipitable counts were applied to each lane and the proteins were separated by SDS-PAGE. The gels were dried and then exposed to films (A and B). The protein bands alphabetized in (B) were quantified using a BAS2000A image analyzer and the relative rates of synthesis are shown in (C).