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ATP-triggered rotational analysis of group II chaperonins in the θ direction.

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posted on 2013-05-29, 02:26 authored by Hiroshi Sekiguchi, Ayumi Nakagawa, Kazuki Moriya, Koki Makabe, Kouhei Ichiyanagi, Shunsuke Nozawa, Tokushi Sato, Shin-ichi Adachi, Kunihiro Kuwajima, Masafumi Yohda, Yuji C. Sasaki

(A) Time-series histograms of the absolute angular displacement in the θ direction per frame (36 ms). (B) Tryptophan fluorescence changes for a group II chaperonin (TKS1-Cpn L265W) in a mixture of ATP, as measured with a stopped-flow spectrofluorometer. (C) Mean square angular displacement (MSD) in the θ direction as a function of time interval in the presence of 0 mM ATP, 2 mM ATP, or 1 mM ATP-AlFx.

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