ASK1 deficiency does not protect from palmitate- or cytokines-induced beta-cell dysfunction.

(A) Insulin secretion in response to 2 and 20 mM glucose in MIN6 shCTL and shASK1cells pre-incubated during 24 h with 20 mM glucose +/− 0.33 mM palmitate. (B) Insulin secretion in response to 2.8 and 16.7 mM glucose in WT and ASK1-KO islets pre-incubated during 24 h with 16.7 mM glucose +/− 0.5 mM palmitate. Insulin secretion in response to 2.8 and 16.7 mM glucose in WT and ASK1-KO islets treated with cytokines during 6 h (C) and 24 h (D). (E) Total insulin content in glucose in WT and ASK1-KO islets treated with cytokines during 6 h and 24 h. Results are mean ± SEM of 3 to 6 independent experiments. *p<0.05, **p<0.01 and ***p<0.001 compared to low glucose condition. $$p<0.01 and $$$p<0.001 compared to high glucose in control conditions.