<i>AGL36</i> is only expressed from the maternal genome.

<p>(A) Real-time PCR analysis showing <i>AGL36</i> expression in L<i>er</i> x <i>cdka;1</i> relative to L<i>er</i> x Col seeds 3 DAP. Gray bars represent L<i>er</i> x Col expression levels, black bars represent the L<i>er</i> x <i>cdka;1</i> expression levels. Left section: <i>AGL36</i> normalized to <i>ACT11</i> levels. Right section: <i>AGL36</i> normalized to <i>GAPA</i> levels. Average values from three independent biological replicas are shown. Error bars indicate standard deviation (STDEV). (B) Schematic overview of <i>AGL36</i> SNP analysis. The presence of a SNP between Col and L<i>er</i> ecotypes (C-T conversion respectively) allows the amplified <i>AGL36</i> cDNA PCR product from the Col ecotype to be digested with <i>Alw</i>NI restriction enzyme, while the L<i>er</i> ecotype remains undigested. (C) <i>AGL36</i> is maternally expressed. Seeds obtained from Col x L<i>er</i> and L<i>er</i> x Col crosses were harvested at 3 DAP followed by <i>AGL36</i> RT-PCR, <i>Alw</i>NI digestion and subsequent Bioanalyzer analysis. Genomic Col and L<i>er</i> were included as controls (Left section, first two lanes). Digestion products of two independent biological replicas of maternal Col x L<i>er</i> pollen crosses produced only Col bands, indicating maternal expression (Middle section). Similarly, the digestion products of two independent biological replicas of maternal L<i>er</i> x Col pollen produced only L<i>er</i> bands, indicating maternal expression (Left section). The intensities of the bands are represented as concentrations (nmol/L), and create a basis for comparison. 100 ng DNA was used as template for each PCR reaction.</p>