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ADAM17 co-precipitates with α5β1 integrin and dissociates from the integrin during GPCR stimulation.

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posted on 20.02.2013, 07:59 by Pal Gooz, Yujing Dang, Shigeki Higashiyama, Waleed O. Twal, Courtney J. Haycraft, Monika Gooz

(A) Control (−) and 1 µM 5-HT stimulated (+) mesangial cell lysates were immunoprecipitated (IP) with either ADAM17 antibody or Ig control (Ig C), resolved on 3–8% Tris-acetate gel and probed for the presence of β1 integrin and α5 integrin by Western blotting. (B) ADAM17 blot shows that equal amount of ADAM17 were precipitated from each sample. Arrows point to specific and non-specific (NS) bands. One representative example out of four experiments is shown.