ΔGCN5-A parasites fail to up-regulate developmental genes in response to alkaline pH.

Wild-type (WT), ΔGCN5-A (KO), or complemented ΔGCN5-A (KO-C) parasites were incubated in control (pH 7.0) or alkaline media (pH 8.2) for four days. The levels of mRNA expression for stress-induced bradyzoite genes BAG1 (A) and LDH2 (B) or the constitutive gene actin, ACT1, (C) were monitored by quantitative real-time PCR. Error bars indicate the S.D. D. ChIP of FLAG-tagged TgGCN5-A performed as described in Fig. 5, but using primers for BAG1 and LDH2 (Table S6). Results are represented as a ratio of ChIP/Input DNA. P value for BAG1 sample = 0.02, P value for LDH2 sample = 0.01 (Student's t-test).