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5-HT and 5-HT-biotin localize to α-actin and are incorporated into proteins.

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posted on 21.02.2013, 05:52 by Stephanie W. Watts, Jessica R. C. Priestley, Janice M. Thompson

A. Immunocytochemistry of aortic smooth muscle cells incubated with exogenous 5-HT (12.7 µM; left) or 5-HT biotin (12.7 µM; right) and α-actin for 1 hour prior to fixation and visualization using an antirabbit fluorescent secondary (for 5-HT) or streptavidin-conjugated secondary (for 5-HT biotin). Representative of four different aortic explants. B. Effect of cystamine (10 mM) on 5-HT-biotin localization in aortic smooth muscle cells. Representative of four different aortic explants.

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