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25-kDa ThTPase activities in the brain and cultured cell lines from humans and mice.

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posted on 25.10.2010, 00:54 by Marjorie Gangolf, Jan Czerniecki, Marc Radermecker, Olivier Detry, Michelle Nisolle, Caroline Jouan, Didier Martin, Frédéric Chantraine, Bernard Lakaye, Pierre Wins, Thierry Grisar, Lucien Bettendorff

(A) ThTPase specific activities measured at pH 8.5, at 37°C and at a Mg2+ concentration of 10 mM (means ± SD, n = 3) in mouse and human brain and cell lines. (B) pH profile of ThTPase activity from various sources (means ± SD, n = 2). The buffers (50 mM final concentration) used were: Pipes (pH 6.5), Mops (pH 7.1), Hepes (pH 7.5), Taps (pH 8.0), Bis-Tris-Propane (pH 8.5) and Ches (pH 9.1 and 9.5).