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21-BD induces apoptosis in HeLa and CHO-K1 cells.

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posted on 07.10.2014 by Sayonarah C. Rocha, Marco T. C. Pessoa, Luiza D. R. Neves, Silmara L. G. Alves, Luciana M. Silva, Herica L. Santos, Soraya M. F. Oliveira, Alex G. Taranto, Moacyr Comar, Isabella V. Gomes, Fabio V. Santos, Natasha Paixão, Luis E. M. Quintas, François Noël, Antonio F. Pereira, Ana C. S. C. Tessis, Natalia L. S. Gomes, Otacilio C. Moreira, Ruth Rincon-Heredia, Fernando P. Varotti, Gustavo Blanco, Jose A. F. P. Villar, Rubén G. Contreras, Leandro A. Barbosa

(A) Score value obtained from the comet assay of CHO-K1 cells incubated 24 h with 21-BD at different concentrations (red circles). (B) Micronucleated cells percentage of CHO-K1 cultures incubated with 21-BD at different concentrations for 24 h. A 24 h incubation with 0.4 mM Methyl methanesulfonate (MMS) was used as a control (A, B, blue circles). (C) Apoptotic and necrotic HeLa cells after 24 h of incubation in control media (white bars), media with 50 µM 21-BD (red bars) or 2 µM digoxin (green bars) for 24 h. Apoptosis and necrosis were detected by flow cytometry ussing an annexin-V translocation assay and the incorporation of propidium iodide in to the nucleus, respectively. P<0.01.