15d-PGJ2, but not rosiglitazone, suppressed cytokine production, degranulation and cytotoxicity functions of Vδ2 T cell.
(A) Freshly isolated PBMC contained 1–10% of Vδ2 T cells (left panel); after 10 to 14 days of culture with IPP plus IL-2, the percentage of Vδ2 T cells was more than 90% (right panel). (B, C and D) Vδ2 T cells were treated with 15d-PGJ2 or rosiglitazone at various concentrations for 1 hour and then washed and stimulated with IPP (50 µM). After stimulating for 4 hours, the levels of IFN-γ (B) or TNF-α (C) in cell-free supernatant were detected by antigen capture ELISA. The experiments were done in triplicate and statistical tests compared drug and vehicle (DMSO). CD107a expression (D) was analyzed by flow cytometry. (E and F) Vδ2 T cells were pretreated with 15d-PGJ2 at various concentrations for 1 hour. The cytotoxicity of Vδ2 T cell against Daudi (E) or TU167 (F) was evaluated at different E∶T ratios in triplicate. The statistical significance of specific lysis compared with a drug vehicle (DMSO) control at E∶T = 5∶1 was analyzed. (G and H) Vδ2 T cells were pretreated with rosiglitazone at various concentrations for 1 hour. The cytotoxicity of Vδ2 T cell against Daudi (G) or TU167 (H) was evaluated at different E∶T ratios in triplicate. (I) PBMC was stimulated with PMA (10 ng/ml) and ionomycin (1 µM) for 4 h. IL-17 production in different cell type was detected by flow cytometry. *, P<0.05; **, P<0.01; ***, P<0.001. Data are representative of three independent experiments using different donors.