Western blot images of α, β, γ-globin proteins and HBG1_HiBiT-tagged protein expression induced by pomalidomide treatment of HUDEP2_HBG1_HiBiT cells for 6 days.
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posted on 2024-11-06, 18:29 authored by Jian-Ping Yang, Rachel Toughiri, Anshu P. Gounder, Dan Scheibe, Matt Petrus, Sarah J. Fink, Sebastien Vallee, Jon Kenniston, Nikolaos Papaioannou, Steve Langston, Narender R. Gavva, Shane R. HormanGAPDH was used as the loading control. Lane 1 (P): parental HUDEP2 cells; Lane 2 (CM): HUDEP2_HBG1_HiBiT cells cultured in expansion media; Lane 3: HUDEP2_HBG1_HiBiT cells in differentiation media with 0.05% DMSO and without pomalidomide. Lane 4–7: HUDEP2_HBG1_HiBiT cells in differentiation media with various concentrations of pomalidomide.
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viable therapeutic approachsmall molecule agonistssickle cell diseaseseveral transcriptional repressorsreporter cell linemodulating small moleculeshudep2 cell linec )- terminusachieved clinical stageparental hudep2 cellshuman primary cd34induced hbf expressionenhance hbf expressionfetal hemoglobin expressiondiv >< pnovel hbf inducersfetal hemoglobinprogenitor cellsnovel therapeuticshuman umbilicalinduce hbfus foodtranslational naturetop hitsstudies demonstratescreening platformhspcs ).hibit peptidehematopoietic stemhbg1 globin proteingenetically tagginggene locusfda ).drug administrationchemogenomic screen10 compounds
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