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Western blot assay to detect vascular endothelial growth factor (VEGF) expression

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posted on 31.12.2011, 00:01 by Eulalia de la Torre, Lilia Davel, María A Jasnis, Tomomi Gotoh, Eugenia Sacerdote de Lustig, María E Sales

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Taken from "Muscarinic receptors participation in angiogenic response induced by macrophages from mammary adenocarcinoma-bearing mice"

Breast Cancer Research 2005;7(3):R345-R352.

Published online 4 Mar 2005


Copyright © 2005 de la Torre et al.; licensee BioMed Central Ltd.

Expression in normal macrophages (NMps) and macrophages from 7-day LMM3 tumor-bearing mice (TMps). TMps were treated with carbachol (CARB) (100 nM) in the absence or presence of 1 μM pirenzepine (PIR), methoctramine (MET) or 4-DAMP. Lane C, control (TMps without treatment). Bands were quantified in optical density units per square millimeter (OD/mm). The molecular mass indicated at the left corresponds to the VEGF isoform detected. One representative experiment of three is shown.


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