Western blot analysis of thymidylate synthase and ternary complex
figure
posted on 2011-12-30, 15:15 authored by Wainer Zoli, Paola Ulivi, Anna Tesei, Francesco Fabbri, Marco Rosetti, Roberta Maltoni, Donata Casadei Giunchi, Luca Ricotti, Giovanni Brigliadori, Ivan Vannini, Dino AmadoriCopyright information:
Taken from "Addition of 5-fluorouracil to doxorubicin-paclitaxel sequence increases caspase-dependent apoptosis in breast cancer cell lines"
Breast Cancer Research 2005;7(5):R681-R689.
Published online 22 Jun 2005
PMCID:PMC1242133.
Copyright © 2005 Zoli et al.; licensee BioMed Central Ltd.
Untreated cells; 5-FU (1 μg/ml); Dox (0.1 μg/ml)→Pacl (0.1 μg/ml); Dox (0.1 μg/ml)→Pacl (0.1 μg/ml)→48-h washout→5-FU (1 μg/ml). 50 μg of protein were loaded for the controls and treated samples.