Western Blot analysis of msfGFP-tagged RNase E truncated or mutated variants.

Anti-GFP (for probing RNase E proteins) and anti-OprF (loading control) Western Blot were performed from crude lysates of strains expressing one of the following msfGFP-tagged RNase E variants from the native locus and promoter, replacing the WT copy.

L: Protein ladder; 1: RNase E-msfGFP; 2: RNase E^AR4mut-msfGFP; 3: RNase E^AR1mut-msfGFP; 4: RNase E^REERmut-msfGFP; 5: RNase E^AR1+AR4mut-msfGFP; 6: RNase E^{AR1+AR4+REERmut}-msfGFP; 7: RNase E^1-588-msfGFP; 8: RNase E^1-529-msfGFP; 9: RNase E-msfGFP no Rifampicin (DMSO only); 10: RNase E-msfGFP + Rifampicin 100 μg/mL (20 mg/mL stock in DMSO).

(EPS)