Vital staining FDA/PI showed increased H2O2 tolerance as a result of incubation with TAT-fused proteins.
SH-SY5Y cells in suspension (2 x 106) were simultaneously treated with 3 μM TAT-LepHO and TAT-LepFNR or left in serum free medium for 1 h and then exposed to 1 mM H2O2 for another hour. Cell pellets were washed with PBS buffer and resuspended in 500 μl of staining medium (serum free DMEM-F12, 2 μg/mL Fluorescein Diacetate, 1.2 μg/mL Propidium Iodide). n = 3, quantification was made using cell counting software (ImageJ) on 3 image fields of each independent experiment. *p<0.05 vs. CON (control) cells; **p<0.01 vs. CON (control) cells; #p<0.05 vs. H2O2 alone-treated cells. Scale bar first column = 50 μm; scale bar second column = 20 μm.