Viscosity changes of glucommanan during the incubation with two H. irregulare LPMOs using ascorbic acid as reducing agent.

The dynamic viscosity was monitored for 30 min at 30°C, pH 5.0, in a falling-ball capillary viscometer with 1 mg/ml glucomannan, 1 uM LPMO and 1 mM ascorbic acid. NcLPMO9C, a LPMO known to oxidatively cleave glucomannan, was used as a positive control, and a negative control containing only glucomannan (GM) and ascorbic acid (AA) was included.