Verification of the utility of the crystal violet (CV) assay for measuring cell number

Copyright information:

Taken from "Membrane estrogen receptor-α levels in MCF-7 breast cancer cells predict cAMP and proliferation responses"

Breast Cancer Research 2004;7(1):R101-R112.

Published online 24 Nov 2004

PMCID:PMC1064104.

Copyright © 2004 Zivadinovic et al., licensee BioMed Central Ltd.

Different numbers of MCF-7 cells enriched for membrane estrogen receptor-α (mER) and MCF-7 cells depleted for membrane estrogen receptor-α (mER; 1000–7000/well) were plated, fixed with 2% paraformaldehyde/0.1% glutaraldehyde, and then quantified via the CV assay at 590 nm absorbance. Different numbers of mERcells were assessed in parallel with CV and MTT assays to demonstrate a linear correlation between these assays. The values are means for 28 samples ± standard error.